An effective combined-enhancer for the PCR amplification of GC-rich longer DNA sequences

摘要：

DNA manipulation technology is facing more challenges in the postgenomics era. The amplification of GC-rich longer DNA fragments PCR more difficult than others. through more and more enhancers have been investigated for their potential implications in developing better gene technology. In this study, we reported a beneficial effect of combined-enhancer in enhancing the specificity and total efficiency of GC-rich（GC％≥65％） longer DNA fragments PCR. Combined-enhancer is a concentration-dependent combination of 5.5M betaine,0.5u pfu enzyme, 1.5mM the mixture of 1,2-propylene glycol and ethylene glycol (the ration of 1,2-propylene glycol and ethylene glycol=1:1)in 25 μL PCR condition. The combined-enhancer broadly enhanced the quantitative and/or qualitative output of GC-rich（GC％≥65）longer DNA fragments PCRs.

关键词： combined-enhancer PCR high GC longer fragment

作者: Fang Liu Wenze Zhu Zhizhou Zhang

作者单位: Teda Bic-X Center for systems Bio Tehnoylgy,Tianjin University of Science andTechnology,Tianjin,3004 College of Biotechnology,TianjinUniversity of science and Technology,Tianjin,300457,china

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-6

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）