Preliminary Study on the Ezpression of BGT gene in Transgenic Birch
In this paper, Southern blot analysis of the transgenic birch (Betula platyphylla Suk.) plants indicated that the the copy number of bgt varies from one to four. Of which 18.2 percent were single copy. Transcriptional expression of bgt gene in transgenic birch plants was analyzed by Northern blot. TGS has been found in four transgenic lines with one, three and four copies of bgt. Bgt gene was inserted into the prokaryotic vector pET 28a and transferred into E.coli BL21 (DE3) for expression of BGT fusion protein. After overnight cleavage with Thrombin and purification by immobilized metal ion affinity chromatography, the final BGT proteins were obtained with purity of 90％. The ELISA result showed that the BGT protein expression level in the transgenic birch plants ranged from 0.000％ to 0.283％ of total soluble protein. No significant correlation was found between copy number and expression level of bgt gene.
Betula platyphylla Transgene Copy number ELISA
Fan-Suo ZENG Hong-Cui ZHAO Ying XIN Ya-Guang ZHAN Feng-Hui QI
College of Life Science,Northeast Forestry University,Harbin 150040,China Key Laboratory of Forest T College of Life Science,Northeast Forestry University,Harbin 150040,China College of Forestry,Northeast Forestry University,Harbin 150040,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）