The proliferative inhibition and apoptotic mechanism of Saikosaponin D in human non-small cell lung cancer A549 cells
Saikosaponin D is a saponin extract derived from several species of Bupleurum (Umbelliferae), which is used for the treatment of various liver diseases in traditional Chinese medicine. In this study, we report that Saikosaponin D inhibits the cell growth of human lung cancer cell line A549 and provide a molecular understanding of this effect. The results showed that Saikosaponin D inhibited the proliferation of A549 by inducing apoptosis and blocking cell cycle progression in the G1 phase. ELISA assay showed that Saikosaponin D significantly increased the expression of p53 and p21/WAF1 protein, contributing to cell cycle arrest. An enhancement in Fas/APO-1 and its two form ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), as well as Bax protein, was responsible for the apoptotic effect induced by Saikosaponin D. Taken together,our study suggests that the induction of p53 and activity of the Fas/FasL apoptotic system may participate in the antiproliferative activity of Saikosaponin D in A549 cells.
Saikosaponin D A549 cells p53 Fas/APO-1 Fas ligand Apoptosis
JIANG Dong YANG Ru-song ZHENG Shi-ying GE Jin-feng ZHAO Jun
Department of Thoracocardiac Surgery,The First Affiliated Hospital of Suzhou University,Suzhou,Jiang Department of Thoracic Surgery,The Thoracic Hospital of Nanjing,Nanjing,Jiangsu,210029,China
国际会议
北京
英文
1-10
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）