Effects of hyperbaric ozygen with free radical antagonists on the ezpression of eztracellular matriz and matriz metalloproteinase -2 in rat liver treated by CCl4
AIM: To investigate the effects of hyperbaric oxygen (HBO) and free radical antagonists (FRA) on the expression of extracellular matrix and matrix metalloproteinase -2 in rat liver treated by CCl4, and to evaluate the preventional effectiveness on liver fibrogenesis. METHODS: Liver fibrosis model was induced by CCl4 in Wistar rats. The rats were randomly divided into control, CCl4, CCl4 with HBO treatment, CCl4 with FRA, and CCl4 with HBO and FRA groups. The rats in the latter three groups were treated with HBO or/and FRA at the 2nd and 8th weeks of the experiment. All the rats were sacrificed at the end of the 8th week. The parameters in sera and tissues were detected. RESULTS: The serum levels of AST and ALT, and the contents of hyaluronate (HA) in the group of CCl4 with HBO and FRA treatment were lower than those in the CCl4 group. The expression of collagen I, collagen IV, MMP-2, TGF-β1 proteins and collagen I, MMP-2 mRNAs in hepatic tissues of the group was also lower than that in the CCl4 group. However, the levels of MMP-2 activity were all higher in three groups treated with HBO or/and FRA than that in the CCl4 group. CONCLUSIONS: This work suggests that hyperbaric oxygen with free radical antagonists can delay the development of liver fibrosis.
Liver fibrosis eztracellular matriz Hepatic stellate cell hyperbaric ozygen free radical antagonists
PS Chen AF Zhang XM Zhang DF Liu XB Li
Department of Pathology School of Basic Medical Science,Southeast University Nanjing,China Department of Internal Medicine Hospital of Chinese Traditional Medicine Zhongshan City,Guangdong,Ch
国际会议
北京
英文
1-5
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）