Relationship between proliferating cell nuclear antigen gene ezpression amount and growth rate of Skeletonema costatum
The partial sequence of Skeletonema costatum proliferating cell nuclear antigen (PCNA) gene was obtained by RTPCR. A 714bp cytochrome b (Cyt b) gene fragment of S. costatum was cloned for the first time. Based on the cloned sequences, a FQPCR method was developed to detect the S. costatum PCNA and Cyt b gene, respectively. The above method was applied to study the relationship between the S. costatum growth rate μ(d-1) and the average expression amount of PCNA gene in a single cell. The expression amount of PCNA gene had large variation in different culture phases, and the trend was well consistent with the growth rate, which suggested that the expression amount of PCNA gene correlated well with the cell division, and the PCNA might be a promising indicator for the S. costatum cell proliferation. While the expression amount of Cyt b gene had no obvious variation during different culture phases, which indicated that the Cyt b was a good potential house-keeping gene. Furthermore, using the PCNA gene as the objective gene and the Cyt b gene as the house-keeping gene, a new approch for estimating the S. costatum in-situ growth rate was established by the relative expression quantity (REQ) of the PCNA gene.
red tide alga growth rate PCNA Cyt b gene
He Shan-ying Chen Kun-bai Mi Tie-zhu
College of environmental science and engineering,Zhejiang Gongshang University,Hangzhou,P.R.China Chen Kun-bai College of environmental science and engineering,Zhejiang Gongshang University,Hangzhou College of environmental science and engineering,Ocean University of China Qingdao,P.R.China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）