Physiological Identification of Salt Tolerance in Transgenic Tobacco Ezpressing Genes Related to Plant Trehalose Metabolism
According to the previous results of the molecular identifications of transgenic tobaccos, the representative individuals, CK (non-transgenic), 121 (with pBI121 vector), 1285 (with pGSA1285 vector), T4 (with gene of trehalase, TRE), S1, S5 (with gene of trehalose -6 -phosphate synthase, TPS), P3 (with gene of trehalose -6 -phosphate phosphatase, TPP), a3 (with anti-TRE), i2 (with RNAi-TRE), were selected for identification of salt tolerance, and the chlorophyll content, Malondialdehyde (MDA), relative conductivity, catalase, proline and peroxidase (POD) have been measured after salt stress during this experiment. The results showed that the transgenic tobaccos with anti-TRE and RNAi-TRE grew better than other individuals under 100mmol/L NaCl salt stress conditions, T4s MDA was observably higher than that of other individuals and i2POD was markedly higher than other plants. These indicate that through application the antisense RNA and RNA interference technology to trehalase gene, it is possible to make the receptor trehalose metabolism changes. The weakening of expression of trehalase gene led to the accumulation of trehalose content and the enhancement of salt tolerance in the transgenic tobaccos.
trehalose transgenic tobacco geng ezpression salt tolerance
Guo Bei Cao Qinqing Yang Aizhen Liu Yufen
dept.of biotechnology Beijing University of Agriculture Beijing,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）