Cloning,characterization and application of the promoter region of the alkaline protease gene in Bacillus alcalophillus PB92
Promoter fragment of alkaline protease gene was cloned from Bacillus alcalophillus PB92 genome by TAIL-PCR.The fragment was sequenced and analyzed, then submitted GenBank (EU130686).The results showed that it contained several typical promoter charactered regions and two reverse translation frames located in －538 to －370 bp and －275 to -128 bp region.Deletion analysis of the sequence indicated that 414 bp upstream of the TSS exhibited predominant promoter activity, while an 105bp length could serve as this function.Additionally, our data demonstrated that a representative Sectype signal peptide structure presented in PB92 alkaline protease signal peptide.The efficiency of PaprE-AprE signal peptide gene cassette was validated by its driving a plant sweet protein monellin gene highly expression in Bacillus subtilis 1A751.
Bacillus alcalophillus TAIL-PCR alkaline protease gene promoter signal peptide
Kun Chen Yong Jiang Nan Wang Xuegang Luo Fuping Lu Tongcun Zhang
Key Laboratory of Industrial Microbiology,Ministry of Education,College of Biotechnology Tianjin University of Science and Technology Tianjin,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）