The Enhancement of drug sensitivity of human AFP-producing gastric carcinoma cell line FU97 to arsenic triozide Using AFP as targeting siRNA
AFP-producing gastric carcinoma(APGC) are known to have a poorer prognosis and to show a higher incidence of liver metastasis than ordinary gastric carcinoma. Therefore, surgical resection, chemotherapy or radiotherapy is usually not satisfactory. In recent years, effects of arsenic trioxide (As2O3) on cell proliferation and induced apoptosis of solid tumors have been widely studied, while little is known about the APGC. This study was undertaken to delineate the effect of As2O3 on AFP gene expression, the cell growth and apopotosis, and to study the effect of the siRNA targeting AFP gene on the drug sensitivity of APGC cell line FU97 to As2O3. AFP gene sequence-specific siRNA expression vector (AFP-siRNA) and sequence-unrelated control were transfected into FU97. At 6h after transfection, the cells were cultured with 2 μmol/L As2O3,with corresponding untreated cells serving as controls.The growth inhibition rate on FU97 was determined by MTT assay; Apoptosis of FU97 was investigated by DNA gel electrophore; The expressions of AFP,VEGF were determined by realtime-PCR, western blot, ELISA and chemistry luminescence. The combination of AFP-siRNA with arsenic trioxide resulted in significantly inhibitory action on growth of FU97 cells, as well as descending of expression of AFP and VEGF.The effective siRNA targeting AFP can increase the drug sensitivity of the FU97 to arsenic trioxide.
Arsenic triozide AFP FU97 siRNA Apoptosis AFP-producing gastric carcinoma
Yan-Fei Jia Yan Zheng Xiao-Li Ma Zhan-Tao Su Yun-Shan Wang
Central Laboratory,Jinan Central Hospital,Affiliated to Shandong University Jinan,Shandong Province,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）