Ezpression Of Bovine Prochymosin Gene In Lactococcus Lactis

摘要：

Bovine prochymosin(bPC) could act to initiate milk clotting. NICE (Nisin Controlled gene Expression system) in lactococcus lactis was one of the most successful and widely using Grampositive gene expression systems. It was composed by six experiments in this study. They are cloning of bovine preprochymosin gene, electrotransformation in lactococcus lactis, construction of expression vector of bPC gene, detection of mRNA after recombinant bPC transcription, identification of recombinant bPC by SDS-PAGE and Western blot. Through the above method, we successfully constructed expression vector of recombinant bPC in lactococcus lactis by using NICE successfully, as well as achieved its expression and identified to immunocompetence of recombinant bPC.

关键词： bovine prochymosin lactococcus lactis recombinant ezpression

作者: Daqing Sun Xingguang Qu Xiyan Han Yuhan Bi Guanghui Zhang Bin Li Lanxia Qin Yujun Jiang Yujun Jiang

作者单位: National Dairy Engineering & Technical Research Center Northeast Agricultural University Harbin,Chin Key Laboratory of Dairy Science,Ministry of Education Northeast Agricultural University Harbin,China

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-3

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）