Influence of gene dose on in vitro culture responses of purple coneflower (Echinacea purpurea L.)
The main objective for this study was to evaluate the influence of gene dose on culture responses of adventitious buds regeneration in one of the most important medicinal plant purple coneflower.Explants of leaf, petiole and root with same gene composition but of various doses of one set, two sets and four sets of chromosomes were taken from in vitro cultured haploid (2n=1X=11), diploid (2n=2X=22) and tetraploid (2n=4X=44) plantlets and cultured on Murashige and Skoog (MS) basal medium supplemented with different levels of benzyladnine (BA).It was found that explants with heavier chromosome dose need higher BA concentration to achieve higher regeneration frequency.In the experiments, explants of haploid and diploid showed higher regeneration potential at 0.3 mg/l BA where as tetraploid required a higher BA concentration of 0.5 mg/l to accelerate the ability.
in vitro culture organogenesis purple coneflower micropropagation ezplants
Dahanayake Nilanthi Xiao-Lu Chen Fu-Cheng Zhao Yue-Sheng Yang Hong Wu
Genetic Engineering Laboratory,College of Life Sciences,South China Agricultural University,Guangzho Genetic Engineering Laboratory,College of Life Sciences,South China Agricultural University Research Research Center of Medicinal Plants,South China Agricultural University,Guangzhou 510642,P.R.China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）