Ezpression of EV71-VP1 Protein and Preparation of its Polyclonal Antibody

摘要：

Utilized the capsid protein VP1 of Enterovirus 71 from clinical specimens of children with hand-foot-and-mouth disease (HFMD) to prepare the polyclonal antibody (pAb), prevented the viral infection.Methods: RNA got out from the diachorema of the sufferer was amplified by RT-PCR and nested PCR.The viral capsid protein (VP1) cDNA was inserted into the vector PET32a(+), an expression vector PET32a-VP1 was constructed.The VP1 protein was expressed in E Coli.and induced by IPTG.The polyclonal antibody (pAb) against the fusion protein was prepared.ELISA and Western blot methods were used to identify the titer and specificity of the pAb.Results:The VP1 protein and the polyclonal antibody were prepared successfully.Conclusions: The successful expression of the capsid protein VP1 of EV71 and the preparation of the polyclonal antibody can afford experiment foundation for the deep research of the biological function.

关键词： Enterovirus 71 VP1 Hand-foot-andmouth disease polyclonal antibody

作者: Yan Wu Jingjing Gao Sulan Yu ShengJun Wang

作者单位: Department of Immunology,School of Medical Science and Laboratory Medicine,Jiangsu University Zhenjiang,China 212013

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-4

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）