Effect of N-terminal and C-terminal Deletion of Cry1Ie of Bacillus thuringiensis on its Ezpression and Purification

摘要：

Crystal proteins are produced by the gram-positive bacterium Bacillus thuringiensis (Bt) during sporulation. The cry1Ie genes were silent in Bt strains, but can be overexpressed in Escherichia coli BL21 (DE3) in inclusion bodies, and the expressed product was toxic to Plutella xylostella, Ostrinia furnacalis, and soybean pod borer (Leguminivora glycinivorella). In order to know the N-terminal and C-terminal amino acid on the structure formation of Cry1Ie protein, four deletion mutants were constructed and the expression and purification of these mutants were studied.

关键词： Bacillus thuringienesis insecticidal protein Cry1Ie mutant

作者: Huicong Shen Chunlu Zhang Shuyuan Guo Jie Zhang Fuping Song

作者单位: School of Life Science & Technology Beijing Institute of Technology Beijing 100081,China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Ch

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-4

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）