Purification and Some Properties of Superozide Dismutase from Aloe saponaria (Ait) Haw.

摘要：

Superoxide dismutase (SOD) from Aloe saponaria (Ait) Haw was purified to electrophoretic homogeneity by a sequential steps,including of acetone and heat of treatment, DEAE-sepharose and Sephacryl S-200 column chromatography. Three SOD components were obtained, designated as SOD-Ⅰ,SOD-Ⅱ,SOD-Ⅲ with the molecular weight of 46,000D, 35,000D and 40,000D, and isoelectric point of 8.9, 7.6, 7.35 respectively. The three components were consisted of two identical subunits. All three SOD components from A. Saponaria (Ait) Haw were Fe-SOD as confirmed by its sensitivity to KCN and H2O2, elemental analyses and the absorption spectrum analyses.

关键词： aloe (Aloe saponaria (Ait) Haw.) purification properties superozide dismutase (SOD)

作者: Tang Yun-ming Tang Liang-ting Xu Rong-min

作者单位: School of Life Sciences,Southwest China University,Chongqing,China 400715

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-4

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）