DNA Length and Cationic Cofactor Dependent Strand Transfer Activity of HIV-1 Integrase
HIV-1 integrase (IN)-catalyzed strand transfer is a divalent cationic cofactor dependent reaction which involves INDNA interaction. In this work, viral DNA substrates with different lengths were used. The strand transfer activity of IN with these DNA substrates in the presence of Mn2+ and Mg2+ were investigated, as well as some other reaction characters related with IN-DNA interaction. It is found that the strand transfer activity is both divalent cation and viral DNA length dependent. The reaction conditions for the wild type IN are stricter than those of soluble mutant IN. Moreover, IN significantly prefers E strand over A strand as the target strand, and both the recessed and blunt end viral DNA have similar strand transfer activity in the presence of either metal ion. This work provides valuable insight for the understanding of IN-DNA interaction and will be helpful in the anti-IN drug development.
HIV-1 integrase strand transfer IN-DNA interaction
Hong-qiu He Cun-xin Wang Wei-zu Chen
College of Life Science and Bioengineering Beijing University of Technology Beijing,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）