Papain Immobilization on Chitosan-coated Nylon Membrane: Preparation and its Application in Cystatin Separation
Nylon membranes are first hydrolyed with dilute HCl and then treated with chitosan before being used as the affinity carrier. Papain as a ligand has been immobilized on the actived membranes with glutaraldehyde as a crosslinking agent. The factors involving with the activity of immobilized papain, such as concentration of glutaraldehyde, pH, temperature, reaction time, and the amount of added papain have been studied. The results show that the optimum conditions for the preparation of immobilized papain nylon affinity membranes are as follows: pH 9.0, 0.5％ glutaraldehyde solution, the concentration of added papain is 10 mg/ml, the reaction time is 6 h at 45℃. The nylon membrane prepared is then used to purify cystatin from potato juice, and shows that they are high efficiency affinity membranes base for cystatin separation.
Affinity membrane chromatography Chitosan Nylon Papain Cystatin
Hua-Li Nie Sai-Nan Su Hai-Tao Zhang Yong Xue Shu-Bai Li Yu-Ting Zhou Tian-Xiang Chen Li-Min Zhu Chris Branford-white
College of Chemistry,Chemical Engineering and Biotechnology Donghua University Shanghai,201620,China Institute for Health Research and Policy London Metropolitan University London,UK
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）