Microstructured Topography Enhanced the Responsiveness of Voltage-gated Calcium Channels in H945RB.3 Human Neural Progenitor Cells

摘要：

A topographical substrate with a packed polystyrene microbead (1.98±0.20 μm in diameter) array was fabricated for the development of cell-based assay systems targeting voltage-gated calcium channels (VGCCs). We found that the microbead arrayed substrates enhanced the attachment and spreading of the cultured human neural progenitor cells (H945RB.3) as compared to the flat polystyrene surfaces. Microbead arrayed substrates also facilitated the development of higher VGCC responsiveness upon neuronal differentiation than flat substrates. The enhancement of both VGCC responsiveness and cell spreading were most significant until day 14 into differentiation.

关键词： microbead microfabrication confocal topography

作者: Ze-Zhi Wu William S.Kisaalita Lina Wang Yiping Zhao

作者单位: College of Bioengineering Chongqing University Chongqing 400044,P.R.China Faculty of Engineering The Faculty of Engineering The University of Georgia Athens,GA 30602,USA

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-4

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）