A new biochip applied to ezamine the ankylosing spondylitis
In this paper, we introduce a novel real-time polymerase chain reaction (PCR) chip, which integrated the pretreatment of biological sample, the PCR reaction and the real-time fluorescent detection. We have built a dam in the chamber of the chip, and formed a crack underneath it to separate the white blood cell from the whole blood. After the lysis of white blood cells which was separated from the whole blood, the real-time PCR reaction was produced by the integrated micro-heaters and resistance temperature detector (RTD), and the real-time fluorescent measurement was made in the identical chamber. Compared with conventional tubular PCR analysis, this chip-based PCR analysis can obtain more accurate results with smaller amount of samples and reagents. We also used the constructed biochip for the examination of HLA-B27 related to ankylosing spondylitis and the results show that the chip is available for the accurate and rapid analysis of HLA-B27 in the whole blood.
Lab on a chip real-time PCR ankylosing spondylitis
Lei Luo Wei Hou Jun Yu
Department of Electronic Science and Technology,Huazhong University of Science and Technology,Wuhan, National Engineering Research Center for Beijing Biochip Technology,18 Life Science Parkway,Chang Pi
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）