Effects of Enhanced UV-B Radiation on the Cell Mitosis of the Callus in Wheat
In order to study effects of enhanced Ultraviolet-B ( 280nm-320nm) radiation on the cell mitosis, mature embryos of wheat as explant were cultured in the Murashige and Skoog (MS) medium with different concentration of 2，4-D and 6-BA. The results indicated the inducing rate of the mature embryos from wheat on the MS with 2, 4-D 1.0 mg/L was higher and the callus was better than on the other Culture medium. The chromosomal mutation and mitosis of the callus in wheat were studied under the condition of the enhanced UV-B radiation. The results showed that enhanced UV-B radiation inhibited the cell division of callus in wheat, several types of chromosomal aberration such as micronucleus, multi-nucleus, nuclear aberration occurred during the interphase, which may be the results of UV-B inhabiting the DNA replication of the cell to lead to the cell DNA mutation and form the pyrimidine dimer.
wheat UV-B chromosomal mutation
Mei-Ping Zhang Rong Han Yong-Jie Shan Ya-ou Li Juan Wu Wen-Hua Tian Li-Mei Gao
College of life science Shanxi Normal University Lin fen,shan xi 041004,China College of urban and environment science Shanxi Normal University Lin fen,shan xi 041004,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）