Preparation and evaluation of implantable chitosan-collagen-soybean phosphatidylcholine film impregnated with mitomycin C-PLA- nanoparticles
This study was designed to develop and evaluate a chitosan-collagen-soybean phosphatidylcholine (SPC) complex film impregnated with mitomycin C (MMC)-PLA-nanoparticles for treatment of hepatocellular carcinoma in mice. MMC-PLAnanoparticles were prepared by a reverse micelle-solvent evaporation method. PLA-MMC-nanoparticles were dispersed throughout the film. SEM, ATR-FTIR were used to character the properties of the complex film. In vitro, MMC release from the MMC contained complex film (MMC-film) was measured by incubation of the film in phosphate buffered saline with drug analysis by using UV/vis spectrometer at 366 nm. The MMC-film exhibited release characteristics with 8.01％ of MMC being released at 1d and an accumulated drug release over 30 d was 40.32％. In vivo, the MMC-film was implanted at subcutaneous tumor sites of mice and the curative effect was compared with those of drug free complex film. The growth of the tumors were inhibited considerably and dose-dependently by MMC-film (P<0.05). In addition, the post-mortem visual examination did not show any signs of internal inflammation and infection nor were there any evidence of implant encapsulation in any of the animals after 20 d of implantation of the MMC-film or drug free complex film. The results of this work suggest that the chitosan-collagen-SPC complex film has the potential to serve as an effective delivery system for MMC.
Chitosan MitomycinC Tumor Implant Drug delivery
Mei Feng Jing Han Qian Sun Zhenqing Hou Qiqing Zhang
Department of gynecology Fujian Provincial Tumor Hospital Fuzhou,China Research center of biomedical engineering Material of College of Xiamen University
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）