Effects of Mechanical Stretch on growth and metabolism of cardiomyocytes in vitro
Mechanical stimulations are critical factors in regulation activities of cardiomyocytes in vivo and in vitro.Cyclic mechanical stretch is an indispensable factor for cardiomyocytes cultured in vitro and construction of engineered cardiac tissue. Optimized parameter of cyclic stretch is key factor for cardiomyocytes culture in vitro. In this study, neonatal cardiomyocytes were subjected to cyclic stretch by a loading device in vitro. Cardiomyocytes received cyclic stretch at different strain magnitudes and different frequencies or cultured normally. After the treatment of stretching, glucose consumption ratio, lactic acid production ratio and ATPase activity of the cardiomyocytes were measured. Intracellular polymeric β-tubulin expression was analyzed by Western Blot. When the strain magnitude of stretch was 8％ and the frequency was 1 Hz, glucose consumption ratio, lactate production ratio and ATPase activity were all higher than other treatment. Polymeric β-tubulin content increased prominently. In conclusion, the optimized parameter of cyclic stretch favourable for cardiomyocytes metabolism in vitro was 8％ (strain magnitude ) and 1 Hz (frequency), polymeric β-tubulin play a role in cardiomyocytes response to mechanical stretch..
mechanical stretch cardiomyocytes metabolism ATPase tubulin
Yong Guo Yan Wei Xi-zheng Zhang Yu-xian Yan Qiang-cheng Zeng
Tianjin Institute of Medical Equipment,Tianjin,PR China Biology department,Dezhou University Dezhou City,PR China
国际会议
北京
英文
1-5
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）