Ezpression of Caspase-3 Gene in Gastric Adenocarcinoma Cell Line SGC-7901 via Ad-FasL
Objective To investigate the relationship between caspase-3 expression and apoptosis in the gastric adenocarcinoma cell line SGC-7901 transfected with the adenovirus-mediated FasL gene.Methods The human gastric adenocarcinoma cell line SGC-7901 was transfected with the adenovirus-mediated FasL gene.The expression of caspase-3 and apoptotic morphological changes were measured by flow cytometry, TUNEL, RT-PCR, and immunocytochemical assays.Results The expression of caspase-3 was low in the gastric adenocarcinoma cell line SGC-7901, but transfection of these cells with Ad-FasL enhanced the cellular expression of the caspase-3 gene and induced susceptibility to Fas/FasL-mediated apoptosis (25.4％ vs.7.2％, p < 0.001).Apoptosis exhibited a positive relationship with caspase-3 expression (γ=0.647, p < 0.01) Conclusions The down-regulation of caspase-3 expression and resistance to Fas/FasL-mediated apoptosis in gastric adenocarcinoma cells were found to be related to the development and progression of the cells.FasL gene transfer may increase caspase-3 expression in gastric adenocarcinoma cells and enhance apoptosis in them.
membrane glycoproteins caspases gastric neoplasms/pathology oncogenes cell death
ZHENG Shi-ying GE Jin-feng ZHAO Jun JIANG Dong LI Hong
Department of Thoracocardiac Surgery,The First Affiliated Hospital of Suzhou University,Suzhou,Jiangsu,215006,P,R,China
国际会议
北京
英文
1-5
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）