Optimization of mRNA display for identifying peptides that bind with thymidylate synthase RNA

摘要：

In vitro display technologies are powerful tools for isolating polypeptide binders against various target molecules. The mRNA display approach to in vitro protein selection is based upon the puromycin-mediated formation of a covalent bond between an mRNA and its gene product. In this paper, we use mRNA display to isolate peptides that bind thymidylate synghase （TS） RNA with high affinity from a large peptide library (>1013 different sequences). The selection scheme was constructed, and the experimental conditions, including library synthesis, formation and isolation of RNA-peptide fusions were optimized. mRNA display could play a key role in the discovery of peptides with desired functions.

关键词： mRNA display thymidylate synathase selection

作者: Song Yan Zheng Wang Hong-li Lv Zhen Guo Xiu-kun Lin

作者单位: School of Environmental Science and Engineering,Dalian Jiaotong University,Dalian 116028,China Key L Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qi School of Environmental Science and Engineering,Dalian Jiaotong University,Dalian 116028,China

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-4

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）