Study on preliminary eztraction and purification of bioflocculant produced by strain F2
The strain F2 which produce excellent flocculating substance were isolated from soil samples and identified as Rhizobium radiobacter. The isolates F2 produced the flocculating substance during the late logarithmic growth phase and the early stationary growth phase. It was founded that the flocculation activated substances of BF distributed mainly in upper phase and were mainly composed of polysaccharide (83.0％) and protein (17.0％). The flocculation activated substances could be recoveried from the supernatant of the fermented medium by cold acetone precipitation and purified by treating with Sevag reagent. It suggested that the optimum extracting conditions were that concentrating multiple is 1/4, acetone consistency is 100％, acetone quantity is 1:2, precipitating time is 2h, and pH is 7, the rate of extraction for the polysaccharides was 30.2％; the optimum purifying conditions were that Vsample:Vsevag is 2:3, Vchloroform:Vbutanol is 5:2, purifying time is 30 min, the rate of eliminating for the protein was 74.2％. After the preliminary extraction and purification, BF remained high flocculating activity.
Bioflocculant Flocculating ratio Polysaccharide Isolation and eztraction
Wei Wang Fang Ma Jie Xing Jixian Yang
State Key Laboratory of Urban Water Resource and Environment,Harbin,150090,China Department of Municipal and Environmental Engineering,Harbin Institute of Technology
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）