Anti-tumor activities of eztracts from the medicinal plants Pinellia ternata and Pinellia pedatisecta
Objectives:To compare anti-tumor activities of different extracts from the medicinal plants Pinellia ternata and its allied species.Methods: MTT cell proliferation assay was used to evaluate the cytotoxicity of the extracts on Bel-7402, HT-29, Hela cells. FCM assay and DNA fragmentation analysis were used to detect cell apoptosis. Immunohistochemical SABC method was performed for detection of the expression of Caspase-3 in HeLa cells. And the content of succinic acid in the rhizomes of plants was determined by HPLC, which was used to control the quality of these medicinal plants. Results: The active part was mainly composed of the ethyl acetate extracts or the fraction of total organic acid, and components from Rhizoma Pinelliae and Rhizoma Pinellia Pedatisectae were more active than that from Rhizoma Typhonii Flagelliforme. The ethyl acetate extracts from Rhizoma Pinellia Pedatisectae could inhibit Bel-7402 cell growth and induce cell apoptosis in a dosage-dependent manner. A further study showed that the anti-tumor effect of extracts from Rhizoma Penillia Pedatisectae was related to cell cycle arrest in S phase and Caspase-3 induced apoptosis. Conclusion: Rhizoma Pinelliae, Rhizoma Pinellia Pedatisectae and Rhizoma Typhonii Flagelliforme all have anti-tumor activities, while Rhizoma Penillia Pedatisectae showed stronger anti-tumor activities, which could be explained by their induction of cell apoptosis.
Pinellia ternata Penillia pedatisecta Antitumor MTT Apoptosis caspase-3
Juan Li Ke-li Chen Bi-sheng Huang Xiang Lei Xin Yu
Guangxi Institute of Botany,the Chinese Academy of Sciences,Guilin 541006,Guangxi Province,P R China Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription,Hubei College of T The School of Medicine Huangshi Institute of Technology Huangshi 435003,P R China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）