Study on Loop-mediated Isothermal Amplification Assay for Detection of Salmonella in Meat Products

摘要：

A novel nucleic acid amplification method, termed Loop-mediated Isothermal Amplification (LAMP), which amplifies DNA with high specificity, efficiency, andrapidity under isothermal conditions using a set of four specially designed primers and a DNA polymerase with strand displacement activity, may be a valuable tool for the rapid detection ofinfectious diseases. This study designed two pairs of gene-specific primers of conservative invA gene, this study establish the LAMP assay of detection of Salmonella successfully, and detection of Salmonella in meat products by LAMP, which is high specifical and sensitive, also save time for the detection of pathogens in food. So it provides the technology platform and technical support for the general detection organizations to promote this technology.

关键词： Loop-mediated isothermal amplification detection Salmonella meat products

作者: Wang Yu Zhang Wei Yuan Yaowu Ma Xiaoyan Zhang Huiyan Su Xudong Wang Zhenqiang Ma Bo

作者单位: College of Food Science and Technology,Agriculural University of Hebei,Baoding Hebei 071001,China

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-6

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）