Replicative Senescence is Present in Cardiac Microvascular Endothelial Cells
Age-related decrease of angiogenesis in myocardium is a big challenge for us to achieve functional regeneration of infarcted myocardial tissues (MI) in aged peoples. Cardiac microvascular endothelial cells (CMECs) play a key role in cardiac angiogenesis upon the MI. However, whether or not cellular senescence is happening in CMECs has not yet been demonstrated clearly. In the present study, using the in vitro culture system, we found that increases in SA-β-gal positive CMECs was associated with the increases of passage number of these cells. In addition, the telomere length of CMECs was shorter in older passage (passage 44) as compared with younger passage (passage 8). The rate of shortening was estimated as 147bp per passage. Our findings indicated that CMECs were experiencing replicative senescence in vitro. A novel strategy to delay senescence in CMECs will be very helpful to improve the angiogenesis of aged hearts and the regeneration of MI tissues.
cardiac microvascular endothelial cells (CMECs) Replicative Senescence myocardial infarction
Liang Cao Dongqing Cai Xiaotao Shen Yingyu Mao Shaokun Liu John Yeuk-Hon Chan Dongqing Cai Liang Cao Xiaotao Shen Yingyu Mao
Key Laboratory for Regenerative Medicine Ministry of Education Ji Nan University Joint Laboratory fo Department of Biomedical Engineering Ji Nan University Guangzhou,510632 P.R.China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）