Analysis of Relationship between Apoptosis and Changes of Ca2+ in HepG-2 Induced by CSA with Laser Scanning Confocal Technology
Laser scanning confoncal technology was used to study relationship between apoptosis and changes of Ca2+ induced by CSA（ Capparis spinosa L. total alkaloid, CSA） on human heptocarcinoma cell HepG-2. Killing effects of CSA on human heptocarcinoma cell HepG-2 was measured by MTT method, while morphological observation on HepG-2 cells was completed by fluorescence microscope. Apoptosis of HepG-2 cells induced by CSA was measured by flowcytometry. In addition, change of intracellular Ca2+ level of CSA on HepG-2 cells was observed by laser scanning confocal microscope. Results: CSA had obvious cytotoxicity on HepG-2 in a dose-dependent manner, and its IC50 was 162.4μg/ml. CSA could induce characteristic apoptosic morphology of HepG-2 cells, and apoptosis percentage was significantly higher than control one. Migration of cells cycle from S phase to G2 phase had been blocked by CSA. Concentration of Ca2+ in HepG-2 had been increased by CSA, which was positive correlation with drug dosage. CSA had obvious effects of killing and inducing apoptosis on human heptocarcinoma cell HepG-2, and overload of Ca2+ might be involved in the events.
laser scanning confocal technology CSA human heptocarcinoma cell HepG-2 apoptosis overload of Ca2+
JI Yu bin Yu Lei Zhang Xiao min
Center of Research and Development on Life Sciences and Environmental Sciences,Harbin University of Institute of Materia Medica and Postdoctoral Program of Harbin University of Commerce,Harbin 150076, Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin 150076
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）