Microemulsion Sensitized Determination of BSA by Resonance Rayleigh Scattering Method

摘要：

The resonance rayleigh scatterting (RRS) spectrum of 3-(4-methylphenyl)-5-(4-methyl-2-sulfophenylazo) rhodanine and bovine serum albumin (BSA) in the BR (Britton-Robinson) buffer solution at pH 2.3 was studied with the presence of SDS microemulsion. It was found the RRS intensity of M4MRASP and that of BSA is weak respectively, but sharply enhanced when the two compounded. The RRS intensity was strong at the wavelengh range of 380～400 nm and the maximum sacttering intensity was obtained at λex=λem=394 nm. The concerntration of BSA is linear with M4MRASP in the range of 0.01～2.0 μg/mL with a detection limit of 3.52×10-9 g/mL. The method was applied to determination of BSA in real samples with satisfactory result.

关键词： resonance rayleigh scattering bovine serum albumin rhodanine

作者: Jinghua Yu Lei Ge Jiadong Huang Shenguang Ge

作者单位: School of Chemistry and Chemical Engineering,University of Jinan,Jinan,250022 China

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-3

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）