The Ezperimental Study on the Functions of Nonmuscle Myosin Ⅱ in Dividing Mammalian Cells
Myosin Ⅱ was inhibited by 30μM blebbistatin at metaphase and anaphase of NRK cells. Our findings showed that myosin Ⅱ was involved in the karyokinesis and maintenance of cell polarity, but not required in actin accumulation in the furrow. Although there was distinct morphology difference from the control, NRK cells inhibited by blebbistatin completed cytokinesis relative normally. The intercellular bridge dynamics analysis showed that intercellular bridge in absence of myosin Ⅱ thinned more quickly than the controls in early cytokinesis. Cell locomotion mediated by actin which generates active traction force may help to drive furrow ingression of myosin Ⅱ inhibition cells.
NRK cells karyokinesis cytokinesis intercellular bridge myosin Ⅱ
LI Xiaona An Meiwen Wang Li Wu Wenzhou
Applied Mechanics and Biomedical Engineering Taiyuan University of Technology Taiyuan,China
国际会议
北京
英文
1-3
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）