Simultaneous Determination of Five Water-soluble Components of Danshen in Rabbit Plasma and its Application to Pharmacokinetics
A sensitive and specific high-performance liquid chromatography (HPLC)-UV method has been developed for the simultaneous determination of five water-soluble components, Danshensu (DSS), protocatechuic acid (PAC), protocatechuic aldehyde (PAL), salvianolic acid B (SAB) and salvianolic acid A (SAA) of Danshen Injection in rabbits.After liquid-liquid extraction with ethyl acetate, plasma sample was eluted from CAP CELL PAK C18 column with a gradient mobile phase composed of component A: water-dimethylformamide-acetic acid (94:4:2) and B: methanol-dimethyl formamide-acetic acid (94:4:2).The detection wavelength was set at 281nm and flow rate was 1.0mL/lmin.The method described in the present paper showed good linearity over a relative wide range and recoveries of 70.3-94.7％, as well as high intra-and inter-day precisions (CV <15％).The limit of detection for DSS, PAC, PAL, SAB and SAA in plasma were 0.1, 0.05, 0.05, 0.1, 0.2μg/mL, respectively.It could be concluded that this method is sensitive, specific, reproducible and completely meet requirements for pharmacokinetic study of five water-soluble components after intravenous administration of Danshen Injection.
High-performance liquid chromatography Danshen Water-soluble Components harmacokinetics
Jin-Chan Yuan Ji-Hong Yao Guo-Zhu Han Xiao-Wei Hu
Zhongshan college of Dalian Medical University Dalian,China Department of Pharmacology,Dalian Medical University Dalian,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）