Antitumor immunity induced by recombinant vaccine alpha-fetoprotein-heat shock protein 70 complez
To construct a recombinant vaccine alphafetoprotein (AFP)-heat shock protein (HSP70) complex, and study its ability to induce specific cytotoxic T lymphocyte (CTL) response and its protective effect against AFP-producing tumor.Methods: A recombinant vaccine was constructed by conjugating mouse alphafetoprotein to heat shock protein 70.By way of intracutaneous injection, mice were primed and boosted with recombinant vaccine mAFP/HSP70, whereas single mAFP or HSP70 injection as controls.The ELISPOT and ELISA were used to measure the frequency of cells producing the cytokine IFN- in splenocytes and the level of anti-AFP antibody of serum from immunized mice respectively.In vivo tumor challenge were carried out to assess the immune effect of the recombinant vaccine.Results: By recombinant mAFP/HSP70 vaccine immunization, the results of ELISPOT and ELISA showed that the number of splenic cells producing IFN- and the level of anti-AFP antibody of serum were significantly higher in mAFP/HSP70 group than those in mAFP and HSP70 groups (108.50 ∮11.70 IFN- 僴spots/106 cells vs 41.60 ∮10.40 IFN- 僴spots/106 cells, 7.32 ∮3.14 IFN- 僴spots/106 cells, P<0.01; 156.32 ∮10.42 g/mL vs 66.52 ∮7.35 g/mL, 5.73 ∮2.89 g/mL, P<0.01).The tumor volume in mAFP/HSP70 group was significantly smaller than that in mAFP and HSP70 groups (42.44 ∮7.14 mm3 vs 392.23 ∮12.46 mm3, 838.63 ∮ 13.84 mm3, P<0.01).Conclusions: The study further confirmed the function of heat shock protein 70ˇs immune adjuvant.Sequential immunization with recombinant mAFP/HSP70 vaccine could generate effective antitumor immunity on AFP-producing tumor.The recombined mAFP/HSP70 vaccine may be suitable for serving as an immunotherapy for HCC.
heat shock protein70 (HSP70) alpha-fetoprotein (AFP) recomninant vaccine Immunity
Qiaoxia Wang Huanping Lin Xiaoping Ying Lansheng Guo Xiaoping Wang Lijie Zhang Liijun Sun Yongxue Zhou
Department of Pathology Shaanxi University of Chinese Medicine Xianyang 712046,China Qiaoxia Wang,Huanping Lin,Xiaoping Ying,Lansheng Guo Department of Pathology Shaanxi University of C
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）