A Novel Method to Bioaccumulate Calcium in Spirulina
To improve the calcium content of Spirulina and develop a new kind of calcium rich Spirulina, a novel bioaccumulation method, bioaccumulation in photoheterotrophic cultures, was developped to bioaccumulate calcium in Spirulina. To elucidate the mechanism of calcium bioaccumulation of Spirulina maxima in heterotrophic condition, growth and calcium contents of S.maxima in the two photoheterotrophic cultures without NaHCO3 were measured. In the glucose culture, the best growth of S.maxima was found in the glucose concentration of 0.5g/L where the calcium content was about 5 times as high as that in photoautotrophic culture (Zarrouk). In the sodium glycerol phosphate (SGP) culture, the growth was lower than that in Zarrouk culture, but the calcium content was about 17 times higher than that of Zarrouk culture. When adding 0.01g/L CaCL2 to the two photoheterotrophic cultures, the microorganism grew badly, but their calcium contents were about 7 and 10 times as high as those that did not add CaCl2. Evaluation based on the Langmuire Equation showed that the higher calcium contents of S.maxima in the two photoheterotrophic cultures were due to the higher binding affinities between S. maxima cells and Ca2+.
calcium bioaccumulation Spirulina azima photoheterotrophic culture
Jiang Zheng Yahui Gao
Key Lab of Science and Technolgoy for Aquaculture and Food Safety in Fujian Province University,Fish School of Life Sciences Xiamen University Xiamen 361005,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）