Transcription factor AP-2 inhibits survivin ezpression
To elucidate the impacts of member of transcription factor AP-2(α, β or γ) on survivin gene expression in cancer cells and to provide a theoretical base for targeted suppression of survivin gene in tumor cells, the survivin core promoter pLuc-230 and one of AP-2 member expression vectors (α, β or γ) or control empty vector were cotransfected into Hela cells to detect the impacts of AP-2 on the activity of survivin core promoter. Then, the regulation of survivin expression by AP-2 was detected with reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot after transfection for 48 hours. It was shown that AP-2(α or γ) could significantly inhibit the activity of survivin core promoter in Hela cells（p＜0.05）while AP-2β did not show such difference. In addition, AP-2 also partly inhibited survivin expression at the transcription level and translation levels in cancer cells. Thus, Inhibition of survivin expression in Hela cells by AP-2 may open up a new approach for effective regulation of survivin gene and provide a potential base for survivin-targeted cancer therapy.
Survivin transcription factor AP-2 cancer cell
Zhang Mengjie He Meilan Liao Yacheng Sun Ming Li Fengzhi Li Fengzhi
School of Life Science and Technology Tongji University Shanghai,200092,China Department of Pharmacology and Therapeutics Roswell Park Cancer Institute Buffalo NY USA 14263
国际会议
北京
英文
1-3
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）