SNAREs-related Pathways in Rat Brains under Simulated Microgravity Environment
The mechanisms of microgravity effect on the nervous system are largely unknown, but understanding such mechanism becomes increasingly important as space exploration continues and mission duration increases. Rat tail suspension experiment is a common method to simulate the microgravity effect. In this study, the tail suspension model was established firstly. Then the O18-labeling quantitative proteomic strategy was applied to detect the differentially expressed proteins in rats brain striatum. 106 proteins with various functional characteristics were identified. By GO classification, many brain protein classes, like metabolism enzymes, cytoskeleton proteins, antioxidant proteins, signaling proteins, neuron-specific proteins and gliaassociated proteins were found. Using the KOBAS server, many enriched pathways, such as SNAREs, MAPK signaling pathway, Calcium signaling pathway were identified. Among them, the syntaxin, SNAP-25 and VAMP were core components of SNAREs complex, which was the minimal vesicles of membrane fusion and played a key role in synapse plasticity. The alteration of the SNAREs-related proteins may further explain the effects of microgravity on learning and memory function.
SNAREs microgravity proteomics O18-labeling
Gaofei Hu Ying Ma Yun Zhou De Cai Shengyuan Xiao Yulin Deng
School of Life Science and Technology,Beijing Institute of Technology,Beijing,China,100081 State Key School of Life Science and Technology,Beijing Institute of Technology,Beijing,China,100081
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）