Study on bacteria in water and biofilm of a pilot distribution networks
In order to study the bacteria status in water and biofilm and better understand the mechanism of second pollution in distribution networks, a pilot distribution networks was built.Different methods, such as heterotrophic plate counts (HPC), acidine orange direct count (AODC), direct viable counts-nalidixic acid (DVC-N.A.) and direct viable counts-5-cyano-2, 3-ditolyl tetrazolium chloride (DVC-CTC) were compared to get a practicable method to detect bacteria in drinking water.Growth status of biofilm on pipe wall was observed by electron microscope.PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) was applied to reveal bacteria diversity in water and biofilm samples.The results showed that direct viable counts method, such as DVC-N.A.and DVC-CTC.could reflect the real state of bacteria in drinking water, and is more suitable for numerating viable bacteria in drinking water.Bacteria diversity showed by PCR-DGGE indicated even at the same sampling time there are more kinds of bacteria in biofilm sample than in water sample.
heterotrophic plate counts (HPC) direct viable counts (DVC) second pollution bacterial water quality water distribution networks
Qing Wu Xin-hua Zhao
School of Environmental Science and Engineering Tianjin University Tianjin,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）