Effects of ultra high-pressure treatment on the red yeast strain RG-8
The effects of ultra high-pressure (UHP) on the growth and its ability of β-carotene biosynthesis of Rhodotorula glutinis RG-8 were studied. After the treatments of 5 repeated cycles at 300 MPa for 15 min, the barotolerant mutant RG-8-9 was obtained from this strain. The survival ratio of the parent strain RG-8 was increased from 1.40×10-3％ after the treatment of first cycle to 1.47×10-1％ after the treatment of fifth cycle. The biomass of mutant RG-8-9 was 22.8 g/L after 72 h of culture, decreased by 2.6％ compared with 23.4 g/L from the parent strain RG-8, but its β-carotene production could reach 20.6 mg/L, increased by 63.5％ compared with 12.6 mg/L from the parent strain RG-8. The genetic quality of mutant strain RG-8-9 was proved to be stable by experiments. The result of restriction fragment length polymorphism analysis suggested that mutant strain RG-8-9 was likely to change in nucleic acid level, and thus enhanced β-carotene production should be a result of gene mutation induced by UHP.
Ultra high-pressure Rhodotorula glutinis mutation β-carotene biosynthesis
Sui-Lou Wang De-jing Chen Guo-Hong Qi Gui Tang Chen Bo Li Zhi-Ping Yang
Department of Food Science and Safety China Pharmaceutical University Nanjing 210009,Jiangsu,China S Shaanxi Key Laboratory for Resource Biology Shaanxi University of Science and Technology Hanzhong 72 Department of Food Science and Safety China Pharmaceutical University Nanjing 210009,Jiangsu,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）