Bio-effects of ultrasound: Inhibitory effects on ultrasound induced apoptosis by cyclosporin A and fructose supplementation
To reveal the potential molecular interaction between mitochondrial permeability transition (MPT) and ultrasound induced apoptosis, hepatocarcinoma cells, HepG2, were treated by low intensity focused ultrasound in this study. Cyclosporin A (CSA), a special inhibitor of MPT, were pre-incubated with cells before ultrasound treatment, as well as fructose and pyruvate, respectively. Cell viability was measured by trypan blue dye exclusion test. The apoptosis was assessed by phosphatidylserine externalization and DNA fragmentation. The pattern of the decrease in mitochondrial trans-membrane potential (ΔΨm) was determined by flow cytometry. The optimal apoptosis with minimal lysis was attained with ultrasound at 5.0W/cm2 for 1 min. In addition, apoptotic cells increased with increasing incubation time post-treatment and peaked at about 12 h. CSA maintained ΔΨm and reduced ultrasound-induced apoptosis, indicating MPT as the molecular mechanism required for apoptosis induced by ultrasound. Moreover, fructose supplementation also maintained ΔΨm and reduced ultrasoundinduced apoptosis, while pyruvate didnt. It revealed glycolytic products of glycolysis, rather than those produced in tricarboxylic acid (TCA) cycle, were responsible for the reduction of apoptosis.
ultrasound apoptosis mitochondrial permeability transition glycolysis
Yi FENG Ming-Xi Wan
The Key Laboratory of Biomedical Information Engineering of Ministry of Education Department of Biomedical Engineering,Xian Jiaotong University,Xi an,P.R.China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）