Multiplez PCR detection by rapid obtaining pathogens in raw milk with filtration

摘要：

Conventional methods of detecting food-borne pathogens in food are cumbersome and time-consuming. To study a rapid detecting method, investigate the usefulness of a filtration for the concentrate bacteria from raw milk and to establish an accuracy and sensitive multiplex PCR for simultaneous detection of five food-borne pathogens in raw milk. The assay can be completed in about 7h and the limit of detection of all five food-borne pathogens for multiplex PCR was as low as 102 CFU/ml of raw-milk. This study, which increases the accuracy and sensitivity, improves traditional methods and can be applied to the food microorganism detection.

关键词： multiplez PCR raw milk food-borne pathogens filtration rapid detection

作者: Wei Liu Qi Lv Feng Zhao Yuhan Bi Mingna Wang Yujun Jiang Yujun Jiang

作者单位: Key Laboratory of Dairy Science,Ministry of Education Northeast Agricultural University Harbin,China National Dairy Engineering & Technical Research Center Northeast Agricultural University Harbin,Chin

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-3

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）