Detection of Haplosporidium nelsoni in oysters from China coast
Oysters of nineteen cities from China coast were investigated in our study. The localization of Haplosporidium nelsoni-like (MSX) plasmodia was detected by light microscopy. In Situ hybridization of infected tissue section was conducted with the H.nelsoni-specific DNA probe MSX1347. DNA was extracted from the oysters Crassostrea gigas, Crassostrea plicatula, Crassostrea rivularis, Ostrea crenulifera for PCR amplification. MSX-A, MSX-B and Hap-F2, Hap-R2 were applied to amplify the MSX. PCR amplification of genomic DNA with primer pairs MSX-A+B and HAP-F2+R2 yielded the expected products for 573bp, 239bp, respectively. These PCR products were sequenced and the similarities from C. gigas was 99％ in the 16S-like rRNA region of H.nelsoni. Subsequently, MSX was identified in Crassostrea Plicatula. However, MSX was not detected in oysters, C. rivularis, O. crenulifera. Our study demonstrates H. nelsoni in oysters presents between 24 and 40 at north latitude in China. These results approved that H. nelsoni occured in China and the distribution of MSX could be relative to host and latitude.
China Crassostrea gigas Crassostrea Plicatula protozoan parasite Haplosporidium nelsoni histology In Situ hybridisation polymerase chain reaction(PCR)
Zhongwei Wang Xin Lv Yubo Liang
School of Environmental Science and Engineering,Dalian Maritime University,Dalian,China School of Life Science and Technology,Dalian Fisheries University,Dalian,China Department of Marine Environmental Ecology,National Marine Environmental Monitoring Center,Dalian,Ch
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）