A potential useful method for calculating relative safety indez of nanoparticles
Toxicity of nanomaterials has been assessed by a number of ways, but nano-toxicity standards for biosafety or environment safety have not been established worldwide. It is likely that the final “standard approaches shall include several components such as cytotoxicity, animal organ injury and body clearance of nanoparticles, while perturbed DNA replication fidelity resulted from nanomaterials is a particular point worthy of investigation for long-term toxicity assessment. This study reported a useful method to calculate the relative capacities of different nanoparticles to introduce DNA replication errors in the rpsL gene -based assays both in vitro (polymerase chain reaction, PCR) and in vivo. The method also offers a platform to develop various technologies in the context of DNA-nanoparticle interactions.
nanomaterials tozicity assessment DNA replication fidelity
Cenchao Shen Wenjuan Yang Qingdai Liu Hisaji Maki Zhizhou Zhang
Teda Bio-X Centre for Systems Biotechnology,Tianjin University of Science and Technology,Tianjin 300 Nara Institute of Science and Technology,Takayama-cho 8916-5,Ikoma,Nara 630-0101,Japan Teda Bio-X Centre for Systems Biotechnology,Tianjin University of Science and Technology,Tianjin 300
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）