Purification of microcystin-LR by solid-phase eztraction procedure
Solid-phase extraction procedure was successfully used to purify microcystin-LR from cultured cyanobacteria. The method involved collection of cells by flocculation with polyaluminium chloride, extraction of harvested cells by 50％ aqueous methanol, clean-up by liquid-liquid extraction with dichloromethane, and purification by solid-phase extraction. More than 8 mg of microcystin-LR with a purity of 95％ (at 238 nm) was obtained in a single run (recovery, 85％). The present method provides a simple and efficient one step purification eliminating the need to use preparative HPLC.
microcystin pufification solid-phase eztraction liquid-liquid eztraction
Xiaoguo Chen Shenghu Zhang Yang Zhang Bangding Xiao
College of Resources and Environmental Engineering Wuhan University of Technology Wuhan,China Institute of Hydrobiology Chinese Academy of Sciences Wuhan,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）