Responsive proteins To Mn Tozicity in Hippophae rhamnoides L.
Hippophae rhamnoides L. Is uniquely capable of growing well under extreme environmental conditions. In this study, analysis of manganese (Mn) stress-responsive proteins in H. Rhamnoides was conducted wherein greenhouse-grown seedlings were subjected to high Mn stress. Using by proteomic techniques, proteins, extracted from leaves, were analyzed using 2-dimensional electrophoresis (2-DE) and MALDI-TOF MS. Altogether, 55 proteins exhibited changes in abundance under stress. Of these, 13 proteins were identified, including three that disappeared under Mn (a putative ABC transporter ATP-binging protein, a heat shock protein Hslu, and a hypothetical protein XP-515578), seven that were up-regulated (three large subunits of rubisco, a hypothetical protein DSM3645-23351, a putative acyl-CoA dehydrogenase, a nesprin-2, and a J-type co-chaperone HSC20) and three that were only detected under Mn stress (a probable nitrogen regulation protein (NtrX), a 4-hydroxyphenylpyruvate dioxygenase, and an unnamed protein product). These proteins may function in β-oxidation pathways in mitochondria, across membranes transport, abnormal protein removal or prevent protein aggregation arrest, cell division, cytoskeleton stabilization, iron-sulfur cluster assembly and antioxidant substance biosynthesis.
Hippophae rhamnoides Mn Tozicity Protein
Ke Chen Gang Xu
School of Life Science and Engineering Southwest University of Science and Technology Mianyang,Sichu Key Lab of Green Pesticide and Agricultural Bioengineering,Chinese Ministry of Education,Center for
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）