会议专题

A Rapid and Sensitive Chemiluminescence Enzyme Linked Immunosorbent Assay for the Determination of Metolcarb Residue in Agricultural Products

An enzyme linked immunosorbent assay based on polyclonal antibodies with enhanced chemiluminescent (ECL) detection for metolcarb in food is developed. Enzyme tracer dilution and concentration of coating antibody were studied. Under the optimum conditions, the IC50 value and IC15 value of assay were determined to be 5 ng mL.1 and 0.2 ng mL.1 respectively. Determination of metolcarb in orange juice and vegetables was accomplished by simple, rapid and efficient extraction methods. Recoveries of metolcarb from spiked samples ranged from79% to 99%. Validation of the ECL-ELISA was conducted by comparison of results from high performance liquid chromatography (HPLC). The correlation between the data obtained using ECL-ELISA and HPLC was high. Therefore, the ECL-ELISA in this study was suitable for the rapid quantitative determination of metolcarb in agricultural products.

Metolcarb Enzyme linked immunosorbent assay Enhanced chemiluminescence food

Jingwei Sun Shuo Wang Tingting Dong

Key Lab of Food Nutrition and Safety,Ministry of Education Tianjin University of Science and Technology,Tianjin 300457,China

国际会议

The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

北京

英文

1-4

2009-06-11(万方平台首次上网日期,不代表论文的发表时间)