Determination of Tetracycline Residue in Food by Simple Resonance Rayleigh Scattering Method
A novel method for the determination of TC using resonance rayleigh scattering (RRS) technique was established. The resonance rayleigh scattering (RRS) spectra of 3-(4-nitrophenyl)-5-(2-sulfonicphenylazo) rhodanine (4NRASP) with tetracycline (TC) was studied. Experimental result showed that when λex=λem=394 nm, the RRS peak of 4NRASP was greatly enhanced with the increase of TC concentration at a pH of 5.5 Britton-Robinson(B-R) buffer solution and the medium of SDS micro-emulsion. The linear range of the calibration curve for TC was 0.05～3.30 μg·mL-1.The detection limit was 2.95×10-10 g·mL-1.The method has been applied to the determination of the trace amount of TC in food samples with satisfactory results. The reaction mechanism was discussed in this work.
resonance rayleigly scattering tetracycline rhodanine
Shenguang Ge Yuanna Zhu Xiaoliang Cheng Ping Dai Jinghua Yu Ai Gao
School of Chemistry and Chemical Engineering,University of Jinan,Jinan,250022 China School of Material and Science Engineering,University of Jinan,Jinan,250022 China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）