DNA Strand Breaks and DNA-protein Crosslinks of Mast Cell Induced by Butyl Benzyl Phthalate
This experiment was about the effect of Butyl benzyl phthalate on DNA strand breaks and the DNA-protein crosslinks in cells. Mouse mast cells cultured in vitro were exposed to different concentrations of BBP (0、4、20、100μmol·L-1) for 1h. Comet assay and KCl-SDS assay were used to measure the DNA strand breaks and DNA-protein crosslinks. Results showed that, all of the BBP treatment groups (4、20、100μmol·L-1) couldThis experiment was about the effect of Butyl benzyl phthalate on DNA strand breaks and the DNA-protein crosslinks in cells. Mouse mast cells cultured in vitro were exposed to different concentrations of BBP (0、4、20、100μmol·L-1) for 1h. Comet assay and KCl-SDS assay were used to measure the DNA strand breaks and DNA-protein crosslinks. Results showed that, all of the BBP treatment groups (4、20、100μmol·L-1) could cause DNA damage, compared with the control group. But, BBP could induce DNA strand breaks at the lower dose (4μmol·L-1), and could primarily induce DPC at the middle dose (20μmol·L-1) and the higher dose (100μmol·L-1). Cause DNA damage, compared with the control group. But, BBP could induce DNA strand breaks at the lower dose (4μmol·L-1), and could primarily induce DPC at the middle dose (20μmol·L-1) and the higher dose (100μmol·L-1).
butyl benzyl phthalate DNA strand breaks DNAprotein crosslinks
Tang Bei Wu Kai Wu Dan Yuan Jun Lin
Laboratory of Environmental Sciences College of Life Sciences,Huazhong Normal University Wuhan,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）