Study on Eztraction and Purification of Bioflocculant Produced by Bioflocculant-producing Bacterium F6

摘要：

Polysaccharide is the main component of the flocculation activated substances produced by bioflocculantproducing bacterium F6.It could be extracted from the supernatant of the fermented medium by cold acetone precipitation and purified by treating with Sevag reagent. The result showed that the optimum extracting conditions were that concentrating multiple is 1/3, acetone consistency is 100％, acetone quantity is 1:2, precipitating time is 2 h, and pH is 7; the optimum purifying conditions were that Vsample:Vsevag is 2:3, Vchloroform:Vbutanol is 4:1, purifying time is 30 min. After the preliminary extraction and purification, flocculating activity reached 98.89％.

关键词： Bio-flocculants Polysaccharide Eztraction and purification

作者: Jie Xing Jixian Yang Wei Wang Fang Ma

作者单位: State Key Laboratory of Urban Water Resource and Environment,Harbin,150090,China Department of Municipal and Environmental Engineering,Harbin Institute of Technology

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-4

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）