Isolation, Identification and Degradation Characterization of p-Chlorotoluene Degrading Strain
A bacterium(strain ycsd01) capable of growing with p-chlorotoluene(p-CT) as sole carbon and energy sources was isolated from rhizosphere soil in phragmites wetland by using the enrichment culture contaminated with p-CT concentration gradient of 20, 40, 80, 120, 160 and 200 mg·L-1.Morphology, physio-biochemical characteristics and phylogenetic analysis based on 16S rDNA sequence indicate that strain ycsd01, which is Gram-positive, facultative aerobic and mobile, forming single oval spore, clearly belongs to Bacillus licheniformis. The suitable initial p-CT concentration, degradation temperature and pH value for satisfactory degradation were about 120 mg·L-1, 30℃ and pH7.0, for 4d in minimal medium culture fluids at vaccination of 10％. Results could offer useful information for the application of strain ycsd01 in bioremediation or the control of p-chlorotoluene -polluted environment.
p-Chlorotoluene Biogradation 16S rDNA
Zhaoxia Li Cheng Ding Huihui Liu Pan Fan
School of Chemical and Biological Engineering,Yancheng Institute of Technology Yancheng,China College of Food and Biological Engineering,Zhengzhou University of Light Industry Zhengzhou,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）