Microbial community analysis during composting of digested sludge and sawdust by using quinone profile and polymerase chain reaction-denaturing gradient gel electrophoresis
Microbial community structure during the composting of digested sewage sludge and sawdust was analyzed by a combination of quinone profile and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The change in total quinine content (TQ) indicated that microbial biomass reached a peak followed by a decrease, whereas the divergence of quinine (DQ) showed that the microbial community diversity increased continuously during the composting process. The PCR-DGGE clarified community changes at species level and the differences in the microbial communities were also determined. The combination of culture-independent methods revealed the community enough to evaluate the composting process.
Microbial community composting digested sludge sawdust quinone profile PCR-DGGE
Lijuan Yang Mengchun Gao Fangyuan Liang Chao Qin
College of Environmental Science and Engineering,Ocean University of China,OUC,Qingdao 266100,China College of Environmental Science and Engineering,Ocean University of China,OUC,Qingdao 266100,China
国际会议
北京
英文
1-4
2009-06-11(万方平台首次上网日期,不代表论文的发表时间)