Formation of Biomimetic Membranes on Carbozylic Acid Terminated Self-assembled Monolayers For Metrology and Biosensor Applications

摘要：

1.Introduction Biomimetic membranes are synthetic phospholipid mixtures that may mimic the composition of various cell membranes, and are therefore frequently employed to enhance biocompatibility of implants and intracorporeous devices, create a pseudo-physiological environment for enzyme-based biosensors and bioreactors, and to study biological membrane processes.Activity and gating of ion channels, antimicrobial action of membrane lysing peptides, electrochemistry of biological charge transfer can all be studied in a controlled environment when using biomimetic membrane platforms. Moreover, analytical tools such as surface plasmon resonance (SPR) or quartz crystal microbalance (QCM) use biomimetic membrane modified sensor surfaces or chips, and artificial cell designs also rely extensively on these structures. It is therefore crucial to establish protocols to create biomimetic membranes in a reproducible way with well defined and controlled properties. Liposome deposition, where vesicles of phospholipid bilayers deliver the membrane to arbitrary substrates, is frequently used. This method is, however, sensitive to variations of the size and composition of the liposomes, and the physicochemical properties of the surface. While a unifying theory of liposome adhesion, rupture, collapse and concomitant fuse into bilayer membranes is yet to be introduced, we present an empirical study that follows the process of membrane formation from the liposome precursor through the deposition process to the structural characterization of the resulting membranes, for representative mammalian (DMPC) and bacteria -like (DMPC:DMPG 4:1) membranes, on a carboxylic-acid terminated self assembled monolayer, which can be easily created on various substrates and thus can be used to make membrane deposition substrate independent.

关键词： biomimetic membrane quartz crystal microbalance liposome size membrane rafts

作者: Adam Mechler Slavica Praporski Steven M Heaton Stefania Piantavigna Kristopher Hall Maria-lzabel Aguilar Lisandra L Martin

作者单位: School of Chemistry,Monash University,Clayton,VIC 3800,Australia Department of Biochernistry and Molecular Biology,Monash University,Clayton,VIC 3800,Australia

会议类型: 国际会议

会议名称: 18th International Symposium on Fine Chemistry and Functional Polymers,4th IUPAC International Symposium on Novel Materials and Their Synthesis(第18届国际精细化学和功能高分子学术讨论会暨第四届IUPAC 国际新型材料及其制备学术讨论会)

会议地点: 江苏镇江

会议语种:英文

页码: 344-345

在线出版日期: 2008-10-15（万方平台首次上网日期，不代表论文的发表时间）