Changes of Physiology and Biochemistry in Adventitious Root Formation of Castanea moilissima cv. Yanshanhong in Vitro
To analyze the physiological and biochemical changes in adventitious root formation, endogenous hormone content and enzyme activity were measured. Shoot tips of C. mollissima were chosen in vitro to be rapidly micropropagated, inducted to root by two-step method. Shoots were treated with 3.0 mg·L-1 IBA in experimental group while some others without IBA in control group, and the samples were regularly collected and separately measured. The results were as follows: (1) During adventitious root formation, IAA content reduced slightly in the first 3 days, thereafter it rapidly climbed to the peak on the 7th day, and then reduced. ABA content kept steady and less than that of the control groups. CTK and GA3 content descended, and the content of CTK was significantly lower than that of the controls. The curve of the ratio of IAA/ABA was similar with IAA contents, while the ratio of IAA/CTKs curve showed no clear regularity. (2) The activities of IAAO and POD gradually increased in the first 3 days and then descended to minimum on the 7th day, on the contrary, the activity of PPO climbed and reached the peak on the 7th day. (3) The ratio of IAA/ABA and the activity of PPO were the key factors of adventitious root formation of C. mollissima.
in vitro culture culture medium endogenous hormone enzyme IAA/ABA
Guo Su-juan Hou Jing-wei Wang Xiao-feng
The Key Laboratory for Silviculture and Conservation of Ministry of Education Beijing Forestry Unive The Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Educa
国际会议
第四届国际板栗大会(Proceedings of Fourth International Chestnut Symposium)
北京
英文
175-182
2008-09-25（万方平台首次上网日期，不代表论文的发表时间）